现在的位置: 旱区作物逆境生物学国家重点实验室» 固定研究人员» 王晓峰»

王晓峰个人简介

  中文版本

  

  1.基本信息

  王晓峰,男,陕西咸阳人,西北农林科技大学旱区作物逆境生物学国家重点实验室研究人员,园艺学院教授、博士生导师,园艺学院副院长。在西北农业大学园艺系学习,先后获学士和硕士学位,1993年7月留校任教。1999年在西北农业大学获博士学位。2000年至2002年在以色列农业研究中心(Volcani Center)和以色列魏兹曼研究所(Weizmann Institute)做博士后研究,2002年至2010年在美国北卡罗来纳州州立大学(NC State University)从事科研工作。

  现为陕西省百人计划特聘教授,陕西省政府特聘专家,陕西省园艺学会理事,美国高级科学研究学会Sigma XI及美国植物学家学会会员。

  2.研究方向和研究兴趣

  主要从事植物生化和分子生物学的研究及其在园艺作物代谢调控分子育种上的应用, 内容包括植物组织培养及遗传转化,基因克隆和功能分析,植物生长发育过程中的基因表达调控,植物激素信号传导的分子机制,植物信号传导的功能基因组学和蛋白组学等。研究兴趣主要为油菜素受体(BRI1) 及其相关受体(BAK1) 的互作组分及信号传导的分子机理, 膜蛋白受体的生物学功能和相互作用等,并致力于将这些基础研究的成果应用于园艺作物的栽培和育种实践。

  应用免疫沉淀及液相色谱与质谱连用(LC-MS/MS),对BRI1(油菜素受体) 和BAK1(油菜素受体相关激酶受体)的活体磷酸化位点进行了系统的鉴定,首次在植物界建立了完整的受体激酶活体磷酸化图谱。借助单个位点的定向突变对所鉴定的磷酸化位点的生化和生物学功能进行了评估。通过对BRI1和BAK1复合体形成和磷酸化的研究, 以及对BRI1 和BAK1 的信号分解,揭示了油菜素信号传导分子机理的几个重要方面,建立了油菜素早期信号传导模型。近年来在番茄、白菜和马铃薯等蔬菜作物上,研究油菜素内酯受体BRI1磷酸化位点的农艺学价值及其在分子育种上的应用,以期通过人为操控磷酸化位点的磷酸化,精准调控油菜素内酯信号的强度和特异性,进而改良作物的重要农艺性状。

  3. 开设课程

  主讲课程包括《园艺植物生物技术》、《新生研讨课》、《科研基本方法》、《园田技能训练》、《蔬菜栽培学》、《蔬菜学研究进展》等。

  4. 主要研究论文

  1) Qiannan Wang, Shufen Wang, Sufu Gan, Xin Wang, Jianwei Liu, Xiaofeng Wang*. (2016) Role of Specific Phosphorylation Sites of Arabidopsis Brassinosteroid-Insensitive 1 Receptor Kinase in Plant Growth and Development. Journal of Plant Growth Regulation. 35(3): 755-769 .  DOI 10.1007/s00344-016-9580-7

  2) Xin Wang, Dongli Gao, Jinjing Sun, Min Liu, YaoYao Lun, Jianshu Zheng, Shenhao Wang, Qingzhi Cui, Xiaofeng Wang*, Sanwen Huang*. (2016) An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber. Journal of Integrative Plant Biology. doi: 10.1111/jipb.12472.

  3) Shan Zhang, Cheng Li, Qi Li, Qian Nan Wang, Shu Hua Huang, Yan Feng Zhang and Xiao Feng Wang*. (2015)  Functional divergence of BAK1 Genes from Brassica rapa in regulating plant architecture. Genetics and Molecular Research. 14 (4): 14587-14596

  4) Mitra SK, Chen R, Dhandayham M, Wang X, Blackburn RK, Kota U,Goshe MB, Schwarts D, Huber SC, Clouse SD*. (2015) An autophosphorylation site database for leucine-rich repeat receptor-like kinases in Arabidopsis thaliana. Plant Journal:82(6):1042-60.

  5) Thierry Halter, Julia Imkampe, Sara Mazzotta, Michael Wierzba, Sandra Postel, Christoph Bu¨ cherl, Christian Kiefer, Mark Stahl, Delphine Chinchilla, Xiaofeng Wang, Thorsten Nu¨ rnberger, Cyril Zipfel, Steven Clouse, Jan Willem Borst, Sjef Boeren, Sacco C. de Vries,  Frans Tax,  and Birgit Kemmerling*. (2014 ) The Leucine-Rich Repeat Receptor kinase BIR2 is a negative regulator of BAK1 in plant immunity.  Curent Biology. 24(2):134-143.

  6) Vikramjit S. Bajwa, Xiaofeng Wang, R. Kevin Blackburn, Michael B. Goshe, Srijeet K. Mitra, Elisabeth L. Williams, Gerard J. Bishop, Sergei Krasnyanski, George Allen, Steven C. Huber, and Steven D. Clouse*. (2013) Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites. Plant Physiology: 163 (1): 30-42.

  7) Man-Ho Oh, Xiaofeng Wang, Steven D. Clouse, and Steven C. Huber*.(2012) Deactivation of the Arabidopsis BRI1 receptor kinase by autophosphorylation within the glycine-rich loop involved in ATP binding. Proc Natl Acad Sci U S A, 109(1):327-332.

  8) Man-Ho Oh, Xiaofeng Wang, Uma Kota, Michael B. Goshe, Steven D. Clouse, and Steven C. Huber*. (2009) Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of Brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A, 106 (2):658-663

  9) Xiaofeng Wang, Uma Kota, Kai He, Kevin Blackburn, Jia Li, Michael B. Goshe, Steven C. Huber, Steven D. Clouse*. (2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in Brassinosteroid signaling. Developmental Cell, 15(2):220-235

  10) Xiaofeng Wang, Michael B. Goshe, Erik J. Soderblom, Brett S. Phinney, Jason A. Kuchar, Jia Li, Tadao Asami, Shigeo Yoshida, Steven C. Huber, Steven D. Clouse*. (2005) Identification and Functional Analysis of in vivo Phosphorylation Sites of the Arabidopsis BRASSINOSTEROID INSENSITIVE 1 Receptor Kinase. The Plant Cell, 17: 1685-1170

  11) Lian-Hai Fu, Xiao-Feng Wang, Yoram Eyal, Gozal Ben-Hayyim*, et al.(2002) A selenoprotein in the plant kingdom: mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chamydomonas reinhadtii glutathion peroxidase. J. Biol. Chem., 277(29):25983-25991

  5. 联系方式

  通讯地址:陕西杨凌邰城路3号 西北农林科技大学园艺学院

  邮编:712100

  Email: wangxff99@nwsuaf.edu.cn; wangxf9993@yahoo.com

  

  English Vision

  

  Prof. Xiaofeng Wang

  State Key Laboratory of Crop Stress Biology in Arid Areas                                   College of Horticulture Northwest A&F University Yangling, Shaanxi, 712100 P. R. China E-mail: wangxff99@nwsuaf.edu.cn

  

  Education

  07/1999    Ph.D., Plant Molecular Biology and Vegetable Physiology, Dissertation topic: Studies on genetic engineering of salt tolerance in Brassica Oleracea Var. Capitata and B . Napus .

  07/1993    M.S., Crop Genetics and Breeding, Thesis: Genetic research of quantitative traits on oyster mushroom (Pleurotus Ostreatus).

  07/1990  B.S., Horticulture,

  All above degrees were earned in Northwestern University of Agriculture, P. R. China.

  Professional Experience

  2010--present   Professor, Associate Dean of College of Horticulture, Northwest A & F University, Shaanxi, P. R. China

  2007--2010     Research Associate, North Carolina State University, NC, USA.

  2002--2007     Post doctor, North Carolina State University, NC, USA.

  2000--2002     Post doctor, Agricultural Research Organization of Israel (Volcani Center) and Weizmann Institute of Science, Israel

  1999-2000     Associate Professor, Northwestern University of Agriculture, P. R. China

  1993-1999    Assistant Professor, Northwestern University of Agriculture, P. R. China

  Academic Interests

  Brassinosteroids (BRs) are plant steroid hormones that regulate a wide range of developmental and physiological processes. I am interested in the signal transduction pathways through which BRs regulate plant growth and development. To gain a comprehensive understanding of the molecular mechanism of BRs signal transduction pathways and to provide inter-disciplinary training to students, a wide spectrum of approaches, including plant tissue culture,  genetic transformation, gene clones, molecular genetics, biochemistry, proteomics, and cell biology, have been applied. Furthermore, a large effort has been dedicated to molecular breedings of horticultural crops with the latest discoveries in molecular mechanism of BRs signal transduction pathway.

  Courses Offered

  The main courses have been taught: <Biotechnology of Horticulture Plants>, <The Basic Principle of Scientific Research>, < Cultivation Science of Vegetables>, <Seminar of Fresh undergraduates >, < General Vegetable Gardening >, <Advance in Vegetable Science> et al.

  Honors and Awards

  Honored with ‘Hundred of Excellent Experts in Shaanxi Province”

  Selected Publications

  Qiannan Wang, Shufen Wang, Sufu Gan, Xin Wang, Jianwei Liu, Xiaofeng Wang*. (2016) Role of Specific Phosphorylation Sites of Arabidopsis Brassinosteroid-Insensitive 1 Receptor Kinase in Plant Growth and Development. Journal of Plant Growth Regulation. 35(3): 755-769 .  DOI 10.1007/s00344-016-9580-7

  Xin Wang, Dongli Gao, Jinjing Sun, Min Liu, YaoYao Lun, Jianshu Zheng, Shenhao Wang, Qingzhi Cui, Xiaofeng Wang*, Sanwen Huang*. (2016) An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber. Journal of Integrative Plant Biology. doi: 10.1111/jipb.12472.

  Shan Zhang, Cheng Li, Qi Li, Qian Nan Wang, Shu Hua Huang, Yan Feng Zhang and Xiao Feng Wang*. (2015)  Functional divergence of BAK1 Genes from Brassica rapa in regulating plant architecture. Genetics and Molecular Research. 14 (4): 14587-14596

  Mitra SK, Chen R, Dhandayham M, Wang X, Blackburn RK, Kota U,Goshe MB, Schwarts D, Huber SC, Clouse SD*. (2015) An autophosphorylation site database for leucine-rich repeat receptor-like kinases in Arabidopsis thaliana. Plant Journal:82(6):1042-60.

  Thierry Halter, Julia Imkampe, Sara Mazzotta, Michael Wierzba, Sandra Postel, Christoph Bu¨ cherl, Christian Kiefer, Mark Stahl, Delphine Chinchilla, Xiaofeng Wang, Thorsten Nu¨ rnberger, Cyril Zipfel, Steven Clouse, Jan Willem Borst, Sjef Boeren, Sacco C. de Vries,  Frans Tax, and Birgit Kemmerling*. (2014 ) The Leucine-Rich Repeat Receptor kinase BIR2 is a negative regulator of BAK1 in plant immunity. Current Biology. 24(2):134-143.

  Vikramjit S. Bajwa, Xiaofeng Wang, R. Kevin Blackburn, Michael B. Goshe, Srijeet K. Mitra, Elisabeth L. Williams, Gerard J. Bishop, Sergei Krasnyanski, George Allen, Steven C. Huber, and Steven D. Clouse*. (2013) Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites. Plant Physiology: 163 (1): 30-42.

  Man-Ho Oh, Xiaofeng Wang, Steven D. Clouse, and Steven C. Huber*.(2012) Deactivation of the Arabidopsis BRI1 receptor kinase by autophosphorylation within the glycine-rich loop involved in ATP binding. Proc Natl Acad Sci U S A, 109(1):327-332.

  Man-Ho Oh, Xiaofeng Wang, Uma Kota, Michael B. Goshe, Steven D. Clouse, and Steven C. Huber*. (2009) Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of Brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A, 106 (2): 658-663

  Xiaofeng Wang, Uma Kota, Kai He, Kevin Blackburn, Jia Li, Michael B. Goshe, Steven C. Huber, Steven D. Clouse*. (2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in Brassinosteroid signaling. Developmental Cell, 15(2):220-235

  Xiaofeng Wang, Michael B. Goshe, Erik J. Soderblom, Brett S. Phinney, Jason A. Kuchar, Jia Li, Tadao Asami, Shigeo Yoshida, Steven C. Huber, Steven D. Clouse*. (2005) Identification and Functional Analysis of in vivo Phosphorylation Sites of the Arabidopsis BRASSINOSTEROID INSENSITIVE 1 Receptor Kinase. The Plant Cell, 17: 1685-1703

  Hashimul Ehsan, William Keith Ray, Brett Phinney, Xiaofeng Wang, Steven C. Huber, Steven D. Clouse*. (2005) Interaction of Arabidopsis BRASSINOSTEROID- INSENSITIVE 1 receptor kinase with a homolog of mammalian TGF-β Receptor Interacting Protein. The Plant Journal, 43: 251-261

  Lian-Hai Fu, Xiao-Feng Wang, Yoram Eyal, Gozal Ben-Hayyim*, et al. (2002) A selenoprotein in the plant kingdom: mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chamydomonas reinhadtii glutathion peroxidase. J. Biol. Chem., 277(29): 25983-25991

  Contact information

  Address: #3 Taicheng Road, Northwest A&F University, College of Horticulture, Yangling, Shaanxi 712100, P. R. China

  Email: wangxff99@nwsuaf.edu.cn; wangxf9993@yahoo.com