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Grapevine VpPR10.1 Functions in Resistance to Plasmopara viticola through Triggering a Cell Death-Like Defence Response by Interacting with VpVDAC3

Hui Ma, Gaoqing Xiang, Zhiqian Li, Yuting Wang, Mengru Dou, Li Su, Xiao Yin, Ruiqi Liu, Yuejin Wang, Yan Xu.

Plant Biotechnology Journal

DOI: 10.1111/pbi.12891

 

 

Abstract: As one of the most serious diseases in grape, downy mildew caused by Plasmopara viticola is a worldwide grape disease. Much effort has been focused on improving susceptible grapevine resistance, and wild resistant grapevine species are important for germplasm improvement of commercial cultivars. Using yeast two-hybrid screen followed by a series of immunoprecipitation experiments, we identified voltage-dependent anion channel 3 (VDAC3) protein from Vitis piasezkii ‘Liuba-8’ as an interacting partner of VpPR10.1 cloned from Vitis pseudoreticulata ‘Baihe-35-1’, which is an important germplasm for its resistance to a range of pathogens. Co-expression of VpPR10.1/VpVDAC3 induced cell death in Nicotiana benthamiana, which accompanied by ROS accumulation. VpPR10.1 transgenic grapevine line showed resistance to P. viticola. We conclude that the VpPR10.1/VpVDAC3 complex is responsible for cell death-mediated defence response to P. viticola in grapevine.