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陈坤明科研团队

  I   科研团队及研究内容

  一、团队名称:作物抗逆细胞分子机制

  Research Team:Cellular and Molecular Mechanism of Plant Stress Tolerance

  二、团队负责人:陈坤明 教授

  

  教育部新世纪优秀人才,第五届国家农业转基因生物安全委员会委员,陕西省细胞生物学会理事,西北农林科技大学细胞生物学学科点点长

  Team Leader: Prof. Kun-Ming Chen

  陈坤明:男,1971年生,理学博士,西北农林科技大学生命科学学院教授,博士生导师,教授委员会委员,细胞生物学学点点长。第五届国家农业转基因生物安全委员会委员,第十四届中国植物结构与生殖专业委员会委员,陕西省细胞生物学学会理事,国家自然科学基金及浙江省等十省市自然科学基金同行评议专家,入选2011年教育部“新世纪优秀人才”。1992年6月毕业于天水师范学院,同年留校任教;2000年6月在兰州大学生命科学学院获植物学理学硕士学位,2003年6月获博士学位,2003年7月至2006年5月在中国科学院植物研究所发育中心从事博士后研究,2007年5月至2009年5月赴芬兰图尔库大学做访问学者。2005年7月至2010年6月在浙江大学农业与生物技术学院工作,任副教授,硕士研究生导师;2010年7月起在西北农林科技大学生命科学学院工作任教授、博士研究生导师。长期从事植物抗逆细胞分子机制和转基因安全等方面的研究工作,先后主持国家自然科学基金面上项目3项,其他省部级项目10余项,参与农业部转基因重大专项2项,近年来在《Plant Physiology》、《Plos Genetics》、《Plant and Cell Physiology》、《BMC Plant Biology》、《Genome Biology and Evolution》等国内外著名学术期刊发表论文50余篇,其中SCI论文30余篇。

  三、团队主要成员:

         

  李文强博士        刘文婷副教授      李绍军副教授

  Team Members: Dr. Wen-Qiang Li, Dr. Wen-Ting Liu, Dr. Shao-Jun Li

  四、主要承担项目:国家自然科学基金面上(No.31270299、32570267)和青年项目(No. 31200148、31401300),教育部新世纪优秀人才项目(NCET-11-0440),陕西省自然科学基金(2016JM3007),重点实验室基本科研业务费重点项目(CSBAAZD1301)等。

  Projects: Major research projects of team members are supported by the National Natural Science Foundation of China (No.31270299, 32570267, 31200148, and 31401300), the Program for New Century Excellent Talents in University of China (NCET-11-0440), the Natural Science Foundation of Shaanxi (2016JM3007), and the Basic Scientific Fund of The State Key Laboratory of Crop Stress Biology for Arid Areas, NWAFU (CSBAAZD1301).

  五、团队主要研究方向:作物抗逆细胞分子机制与调控

  以小麦、水稻等大作物为研究对象,进行作物抗旱、抗盐及抗高低温等非生物胁迫的细胞分子机制、信号调控、以及抗逆相关特异性状基因定位、克隆与功能研究。主要围绕ROS信号途径及其调控关键基因家族如NOX、NADK、RLK等开展研究,以抗逆相关重要基因的克隆、表达分析、分子操纵为切入点,采用正向或反向遗传学的手段,解析作物抗逆尤其是抗旱的分子调控机制,进行抗逆关键基因的挖掘及相关优异种质的创制。

  Research Interests:Plant stress mechanism and regulation

  We are working on plant (mainly wheat and rice) stress physiology and molecular biology, in which the cellular and molecular mechanism, transduction pathways,as well as the cloning and mapping of stress tolerance related genes are addressed to be deeply investigated. Our studies are particularly focusing on ROS signaling and its related regulatory key genes (NOX, NADK, and RLK families) by employing the approaches including map-based gene cloning, transgenic system, and systems biological methods.

  六、团队近期研究进展 Recent Progress of Research:

  自2010年以来,本团队已先后在 Plant Physiology, Plant and Cell Physiology, Genome Biology and Evolution, Physiologia Plantarum , PLoS One 等国际期刊发表SCI论文16篇。

  1. NOX家族抗逆分子机制与ROS信号调控(Molecular mechanisms of NOX family genes in stress tolerance and ROS signaling of rice)

  我们首先在全基因组水平研究了植物NADPH氧化酶(NADPH oxidase,NOX)家族基因的系统进化关系及逆境表达特征,然后克隆了众多与水稻和小麦活性氧(Reactive oxygen species,ROS)代谢相关的基因并通过遗传转化、蛋白互作等手段深入解析了部分基因的功能。该研究系首次系统深入地从分子水平研究水稻NOX家族基因的功能及其与在ROS信号调控中的作用。

  We first identified the NOX family genes and their expression profiles under various stress conditions, and then a lot of genes related to ROS production and metabolism were cloned and investigated deeply by using transgenic systems and protein interaction analysis. Our research is  the first systematical and comprehensive characterization of the functions of the NOX genes and their functioning in ROS signaling of rice.

  

  图1. 植物NOX家族基因系统发育关系与蛋白结构组成

  Fig. 1. Phylogenetic relationships and domain organization of NOX family genes in plants.

  

  图 2. 水稻OsNOX2的亚细胞定位分析

  Fig. 2. Subcellular localization of OsNOX2 in rice.

  2. 小麦NADK家族基因的功能及其抗逆分子机制(Molecular mechanisms of NADK family genes in stress tolerance of wheat)

  本研究我们在系统研究了植物NADK(NAD kinase, NADK)家族基因系统进化关系的基础上,成功克隆到了4个小麦NADK基因的全长并对其功能进行了深入研究,发现TaNADK1和2定位于细胞质,TaNADK3定位于叶绿体,而TaNADK4定位于过氧化物酶体,揭示NADKs广泛参与小麦非生物逆境胁迫响应,并证明TaNADK4与三个HOX家族转录因子有互作关系。

  Our group systematically analyzed the conservation and evolution of NADK gene family and successfully cloned 4 NADK genes in wheat. The further analysis found that TaNADK1 and 2 are localized in cytosol, TaNADK3 is localized in chloroplasts, whereas, TaNADK4 is localized in peroxisome in wheat. The further studies demonstrated that NADK family genes have diverse functions in the plant stress tolerance.

  

  图3. 小麦NADK基因的克隆

  Fig. 3. Cloning of NADK genes in wheat.

  3. 水稻ROS相关RLK家族基因的功能及抗逆分子机制 (The functions of ROS related RLK family genes and their mechanism in stress tolerance of rice)

  本研究我们鉴定到了两个与ROS信号调控相关而功能未知的类受体蛋白激酶(receptor-like protein kinases, RLKs),发现其分别定位于质膜和胞质,分别命名为OsRLK2和OsRLCK267,二者共表达且广泛参与一系列非生物胁迫响应,过表达能够显著提高水稻植株的抗逆性。

  We identified two ROS-related RLKs from rice, namely OsRLK2 and OsRLCK267. It was found taht the two RLKs are localized in the plasma membrane and cytosol in rice, respectively. They exhibited somewhat coexpression characteristics in rice under a series of stress conditions and their overexpression can raise the tolerance of the plants to various abiotic stresses such as drought and salinity.

  

  图4. ROS信号调控相关激酶OsRLCK267互作蛋白的筛选

  Fig. 4. The screening of interaction proteins with OsRLCK267 which is involved in ROS signaling regulation.

  

  图5. 过表达OsRLCK267提高了水稻的抗旱性

  Fig. 5. Overexpression of OsRLCK267 enhanced the drought stress tolerance of rice.

  4. 水稻和小麦突变体库的构建及抗逆基因挖掘 (Establishment of mutant libraries of rice and wheat and identification of the key genes involving stress tolerance)

  利用EMS诱变创建了水稻和小麦突变体库,已获得水稻突变体株系600多个,小麦突变体株系100多个;利用图位克隆技术定位克隆了多个水稻抗逆相关关键基因如 OsCDL1 、 OsWAR1 、 OsGL3 等,并利用遗传转化、系统生物学等手段对其功能进行了深入研究。

  Using EMS mutation, we established two mutant libraries of rice and wheat. More than 600 and 100 mutants have been identified from both the libraries, respectively. Several genes such as OsCDL1, OsWAR1 and OsGL3 , which have important traits in both agronomy and stress tolerance were cloned and deeply investigated using transgenic method and systems biological approaches. 

  

  图6. 水稻突变体库中部分植株性状的突变表型

  Fig. 6. Phenotypes of some rice mutants.

  

  图7. 水稻 OsGL3 基因的克隆与功能分析

  Fig. 7. The cloning and functional analysis of OsGL3 gene.

  

  II 承担的科研项目(Projects)

  自2010年以来,团队共承担国家自然科学基金面上项目3项;国家基金青年项目2项;教育部新世纪优秀人才项目1项;重点实验室科研业务重点项目1项;陕西省自然科学基金项目2项;学校基本科研业务费项目6项。具体如下:

  小麦NADK家族基因参与非生物胁迫应答的分子调控机制研究, 旱区作物逆境生物学国家重点实验室基本科研业务费重点项目,编号:CSBAAZD1301;2013.1-2015.12;主持人:陈坤明;

  水稻OsNOX2抗旱的分子机制及其调控信号研究,国家自然科学基金面上项目,批准号:31270299;2013.1-2016.12;主持人:陈坤明;

  小麦NOX酶家族基因功能及其抗逆的细胞分子机制,2011年教育部新世纪优秀人才支持计划项目,项目编号:NCET-11-0440,2012.1-2014.12;主持人:陈坤明;

  水稻活性氧介导的干旱胁迫响应与忍耐分子生理机制解析,国家自然科学基金面上项目,批准号:30871469; 2009.1-2011.12,主持人:陈坤明;

  水稻GL3基因调控表皮毛发育的遗传和分子机制解析,国家自然科学基金面上项目;批准号:;2016.1-2019.12;主持人:李文强;

  水稻新卷叶基因OsCLD1的克隆与分子调控机制解析,国家自然科学基金青年项目,批准号:31200148;2013.1-2015.12;主持人:李文强;

  小麦干旱胁迫应答基因TaNADK1/2的功能及抗旱作用机制研究,国家自然科学基金青年项目,批准号:31401300;2015.1-2016.12;主持人:李文燕;

  小麦逆境应答转录因子TaMYB1的克隆及功能分析,陕西省自然科学基金面上项目,编号:2016JM3007,2016.1-2017.12;主持人:陈坤明

  水稻D62基因介导GA 与BR信号互作的分子机制,陕西省自然科学基金项目,2014.1-2016.12;主持人:李文强;

  作物NOX酶抗逆的细胞分子机制与遗传转化,西北农林科技大学引进人才科研启动费2010.7-2013.7;主持人:陈坤明

  小麦NADK基因转录调控关键因子鉴定及其胁迫应答机制解析,校农业科技创新专项,批准号:2014YB033;2014.1-2017.1;主持人:李文燕;

  小麦ROS代谢相关DOF转录因子的克隆及其抗逆性分析,校农业科技创新专项批准号:2014YB041;2014.1-2017.1;主持人:刘文婷;

  水稻类受体蛋白激酶LRK2和RLCK267互作蛋白网络构建及抗逆功能分析,校基本科研业务费,批准号:2452015217;2015.8-2015.12;主持人:陈坤明;

  水稻表皮毛调控基因GL3的克隆及其非生物逆境响应机制研究,校基本科研业务费专项,批准号:2015.1-2017.12;主持人:李文强;

  水稻GL3基因的克隆及功能分析,校基本科研业务费培育项目,批准号:2015.8-2015.12;

  小麦同源Os02g0252400基因克隆及其抗逆性分析,陕西省自然科学基金,批准号:2015JQ3075;2015.1-2016.12;主持人:刘文婷。

  

  III 发表的论文(Publications)

  (*通讯作者)

  Shuai-Qi Yang#, Wen-Qiang Li#, Hai Miao, Peng-Fei Gan, Lei Qiao, Yan-Li Chang, Chun-Hai Shi*, Kun-Ming Chen*. REL2 , a gene encoding an unknown function protein which contains DUF630 and DUF632 domains controls leaf rolling in rice. Rice, 2016, 9:37. doi:10.1186/s12284-016-0105-6. (IF2016 = 3.417) (中科院农林科学分类1区)

  Ya-Jing Wang, Xiao-Yong Wei, Xiu-Qing Jing, Yan-Li Chang, Chun-Hong Hu, Xiang Wang, Kun-Ming Chen*. The fundamental role of NOX family proteins in plant immunity and their regulation. International Journal of Molecular Science, 2016, 17: 805; doi:10.3390/ijms17060805. (IF2016 = 3.257) (中科院化学综合分类3 区)

  Yan-Li Chang, Wen-Yan Li, Hai Miao, Shuai-Qi Yang, Ri Li, Xiang Wang, Wen-Qiang Li, Kun-Ming Chen*. Comprehensive genomic analysis and expression profiling of the NOX gene families under abiotic stresses and hormones in plants. Genome Biology and Evolution, 2016, 8(3):791-810. doi: 10.1093/gbe/evw035 (IF2016 = 4.098) (中科院生物分类2区)

  刘文婷,马田田,周春菊,藏潇,李浪金,张宝军,杜威,张炜丽,陈坤明*. 禾本科植物DOF 家族基因对逆境应答的研究进展. 生物工程学报, 2016, DOI: 10.13345/j.cjb.150351

  Wang X, Zhang MM, Wang YJ, Gao YT, Li R, Wang GF, Li WQ, Liu WT, Chen KM*. The plasma membrane NADPH oxidase OsRbohA plays a crucial role in developmental regulation and drought-stress response in rice. Physiologia Plantarum, 2016, 156: 421–443, DOI: 10.1111/ppl.12389. (IF2016 = 3.52)(中科院植物科学分类下的2区)

  Wang X#, Li WY#, Zhang MM, Gao YT, Liu WT, Li WQ, Muhammad I, Chen KM*. Identification and functional analysis of the NADK genes in wheat. Plant Molecular Biology Reporter, 2016, 34: 118-135. DOI: 10.1007/s11105-015-0904-8. (IF2015=2.304)(中科院生化研究方法分类2区)

  Guo W, Chen S, Hussain N, Cong Y, Liang Z, Chen KM*. Magnesium stress signaling in plant: Just a beginning. Plant Signaling & Behavior, 2015, 10:3, e992287.

  Wu D, Chu HY, Jia LX, Chen KM*, Zhao LQ*. A feedback inhibition between nitric oxide and hydrogen peroxide in the heat shock pathway in Arabidopsis seedlings. Plant Growth Regulation, 2015, 75: 503-509. (IF2015=1.672)(中科院植物科学分类下的 3 区)

  Guo W, Cong Y, Hussain N, Wang Y, Liu Z, Jiang L, Liang Z, Chen KM*. The Remodeling of seedling development in response to long-term magnesium toxicity and regulation by ABA-DELLA signaling in Arabidopsis. Plant and Cell Physiology, 2014, 55(10): 1713-1726. (SCI; IF2014="4.978) (中科院植物科学分类下的 2 区)

  Wen-Yan Li, Xiang Wang, Ri Li, Wen-Qiang Li, Kun-Ming Chen*. Genome-wide analysis of the NADK gene family in plants. PLoS ONE, 2014, 9(6): e101051. (SCI; IF2014="3.534) (中科院综合性期刊分类下的 2 区)

  Wang L, Guo Y, Jia L, Chu H, Zhou S, Chen KM, Wu D, Zhao L. Hydrogen peroxide acts upstream of nitric oxide in the heat shock pathway in Arabidopsis seedlings. Plant Physiology, 2014, 164: 2184-2196. (SCI; IF2014="7.394)(中科院植物科学分类下的 1 区)

  苟维超,邓春浩,何俊皓,陈坤明*. 光合细菌产氢研究进展与问题. 可再生能源, 2013, 31(9): 94-101.

  Wang GF, Li WQ, Li WY, Wu GL, Zhou CY, Chen KM*. Characterization of rice NADPH oxidase genes and their expression under various environmental conditions. International Journal of Molecular Science, 2013, 14: 9440-9458. (SCI; IF2013="2.464)(中科院化学综合分类下的 3 区)

  丛悦玺,骆东峰,陈坤明,蒋立希*,郭万里*. 生物镁离子转运体研究进展. 农业生物技术学报, 2012, 20(7): 837-848.

  刘忠丽,丛悦玺,苟维超,王响,陈坤明*. MYB类转录因子在植物细胞生长发育中的作用及其应用. 浙江农业学报, 2012, 24(1): 114-119.

  Haijun Gong, Kunming Chen. The regulatory role of silicon on water relations, photosynthetic gas exchange, and carboxylation activities of wheat leaves in field drought conditions. Acta Physiologiae Plantarum, 2012, 34: 1589-1594 (SCI索引刊物;IF2012=1.639) (中科院植物科学分类下的 3 区)

  Kun-Ming Chen, Hai-Jun Gong. Ascorbate-glutathione related redox regulation in plant stress tolerance. In eds: Anjum NA, Umar S, and Iqbal M; Oxidative Stress in Plants: Causes, Consequences and Tolerance. Published by IK International, New Delhi, India. 2012, pp259-290.

  刘忠丽,丛悦玺,苟维超,王响,陈坤明*. ATMYB123 和 ATKOR1 基因参与拟南芥根发育的调控. 西北植物学报, 2012, 32(1): 0042-0047.

  Wu LL, Liu ZL, Wang JM, Zhou CY, Chen KM*. Morphological, anatomical, and physiological characteristics involved in development of the large culm trait in rice. Australian Journal of Crop Science, 2011, 5(11): 1356-1360. (SCI索引刊物;IF2011=0.899)

  吴丽丽,周丛义,高永生,丛悦玺,陈坤明*,郭万里. 水稻 NADK3 基因的克隆及其遗传转化. 核农学报,2011, 25(5): 0863-0870.

  Kun-Ming Chen, Mirva Piippo, Maija Holmström, Markus Nurmi, Eveliina Aro, Marjaana Suorsa, Eva-Mari Aro. A chloroplast-targeted DnaJ protein AtJ8 is negatively regulated by light and has rapid turnover in darkness. Journal of Plant Physiology, 2011, 168: 1780-1783. (SCI;IF2011=2.677) (中科院植物科学分类下的 3 区)

  周丛义,吴国利,段壮芹,吴丽丽,高永生,陈坤明*. H2O2-NOX系统:一种植物体内重要的发育控制与胁迫响应机制. 植物学报,2010, 45: 615-631

  Kun-Ming Chen, Maija Holmström, Wuttinun Raksajit, Marjaana Suorsa, Mirva Piippo,  Eva-Mari Aro*. Small chloroplast-targeted DnaJ proteins are involved in optimization of photosynthetic reactions in Arabidopsis thaliana . BMC Plant Biology, 2010, 10: 43 doi:10.1186/1471-2229-10-43 (SCI;IF2010=4.03) (中科院植物科学分类下的 2 区)

  Shi CG, Zhu YN, Li YL, Guo WL, Chen KM, Shamsi IH, Hua SJ, Zhong ZF, Zhou WJ, Jiang LX. DNA allelic variations at the loci putatively implicated in seed oil formation among Brassica oilseed cultivars. Molecular Breeding, 2010, 26: 51–64. (SCI;IF2010=2.193)(中科院农艺学分类下的 2 区)

  Duan ZQ, Bai L, Zhao ZG, Zhang GP, Cheng FM, Jiang LX, Chen KM*. Drought-stimulated activity of plasma membrane nicotinamide adenine dinucleotide phosphate oxidase and its catalytic properties in rice. Journal of Integrative Plant Biology, 2009, 51: 1104-1115.  (SCI;IF2009=1.603) (中科院生化与分子生物学分类下的 3 区)

  Pak H, Guo Y, Chen MX, Chen KM, Li YL, Hua SJ, Shamsi I, Meng HB, Shi CG, Jiang LX*. The effect of exogenous methyl jasmonate on the flowering time, floral organ morphology, and transcript levels of a group of genes implicated in the development of oilseed rape flowers ( Brassica napus L.). Planta, 2009, 231: 79-91. (SCI; IF2009="3.098)(中科院植物科学分类下的 2 区)

  Duan ZQ, Wang JM, Bai L, Zhao ZG, Chen KM*. The alterations in anatomical and chemical characteristics rather than the changes in photosynthetic dynamics and apoplastic transport were involved in brittleness mutation of rice. Journal of Integrative Plant Biology, 2008, 50: 1508-1517. (SCI; IF2008="1.603)" (中科院生化与分子生物学分类下的 3 区)

  Bai L, Duan ZQ, Wang JM, An LZ, Zhao ZG, Chen KM*. Anatomical and chemical characteristics of a rolling leaf mutant of rice and its ecophysiological properties. Rice Science, 2008, 15: 201-208. (核心期刊)

  Chen KM, Wu GL, Wang YH, Tian CT, Šamaj J, Baluška  F, Lin  JX*. The block of intracellular calcium release affects the pollen tube development of Picea wilsonii by changing the deposition of cell wall components. Protoplasma, 2008, 233: 39-49. (SCI 索引刊物; IF2013="2.855)" (中科院植物科学分类下的 4 区)

  Chen KM*, Gong HJ, Wang SM, Zhang CL. Antioxidant defense system in Phragmites communis Trin. ecotypes. Biologia Plantarum, 2007, 51:754-758.(SCI 索引刊物; IF2013="1.692)(中科院植物科学分类下的 3 区)

  Cheng FM, Zhang QF, Zhu HJ, Zhao NC, Wang F, Chen KM, Zhang GP*. The difference in amylase content within a panicle as affected by the panicle morphology of rice cultivars. Journal of Cereal Science, 2007, 46: 49-57. (SCI; IF2013="2.088)(中科院食品科技分类下的 3 区)

  Sun BT, Jing Y, Chen KM, Song LL, Chen FJ, Zhang LX*. Protective effect of nitric oxide on iron deficiency-induced oxidative stress in maize ( Zea mays ). Journal of Plant Physiology, 2007, 164: 536-543. (SCI; IF2013="2.699)" (中科院植物科学分类下的 3 区)

  Chen KM, Wang F, Wang YH, Chen T, Hu YX, Lin JX*. Anatomical and chemical characteristics of foliar vascular bundles in four reed ecotypes adapated to different habitats. Flora, 2006, 201: 555-569. (SCI; IF2013="1.716)" (中科院植物科学分类下的 3 区)

  Chen KM*, Gong HJ, Wang SM, Zheng WJ,Zhang CL. Regulations of the structure and catalytic property of plasma membrane H+-ATPase involve in adaptation of two reed ( Phragmites communis ) ecotypes to their different habitats. Biologia Plantarum, 2005, 49:513-519.(SCI; IF2013="1.692)(中科院植物科学分类下的 3 区)

  Chen KM*, Gong HJ, Chen GC, Wang SM, Zhang CL. Gradual drought under field condition influences the glutathione metabolism, redox balance and energy supply in spring wheat. Journal of Plant Growth Regulation, 2004, 23: 20-28. (SCI; IF2013="1.99)" (中科院植物科学分类下的 3 区)

  Chen KM, Gong HJ, Wang SM*, Zhang CL. The regulation of the plasma membrane redox system and H+-transport in adaptation of reed ecotypes to their habitats. Biologia Plantarum, 2004, 48: 87-92. (SCI; IF2013="1.692)" (中科院植物科学分类下的 3 区)

  Chen KM, Hai-Jun Gong, Guo-Cang Chen, Suo-Min Wang, Cheng-Lie Zhang. Up-regulation of glutathione metabolism and changes in redox status involved in adaptation of reed ( Phragmites communis ) ecotypes to drought-prone and saline habitats. Journal of Plant Physiology, 2003, 160: 293-301 (SCI; IF2013="2.699)" (中科院植物科学分类下的 3 区)

  Chen KM,Gong HJ,Chen GC,Zhang CL*. ACC and MACC biosynthesis and ethylene production in water-stressed spring wheat. Acta Botanica Sinica, 2002, 44 (7): 775-781. (SCI 索引刊物; IF2013="3.75)" (中科院植物科学分类下的 3 区)

  Gong HJ, Chen KM, Chen GC, Wang SM*, Zhang CL. Effects of silicon on the growth of wheat under drought. Journal of Plant Nutrition, 2003, 26: 1055-1063 (SCI 索引刊物; IF2013="0.526)" (中科院植物科学分类下的 4 区)

  Gong HJ, Chen KM, Chen GC, Wang SM*, Cheng-lie Zhang. Drought stress stimulates p-nitrophenyl phosphate hydrolysis rate of the plasma membrane H+-ATPase from wheat leaves. Plant Growth Regulation, 2003, 40: 139-145 (SCI 索引刊物; IF2013="1.67) (中科院植物科学分类下的 3 区)

  Gong HJ, Chen KM, Chen GC, Zhao ZG, Wang SM*, Zhang CL. Redox system in the plasma membranes of two ecotypes of reed (Phragmites communis Trin.) leaves from different habitats. Colloids and Surfaces B: Biointerfaces, 2003, 32: 163-168. (SCI 索引刊物; IF2013="3.554)(中科院生物材料分类下的 3 区)

  Gong HJ, Zhu XY, Chen KM, Wang SM*, Zhang CL. 2005. Sillicon alleviates oxidative damage of wheat plants in pots under drought. Plant Science, 2005, 169: 313-321. (SCI 索引刊物; IF2013="2.922)" (中科院植物科学分类下的 3 区)

  GONG Hai-jun, CHEN Kun-ming, GAO Yong-sheng, ZHAO Zhi-guang, WANG Suo-min, ZHANG Cheng-lie. Antioxidant system in two ecotypes of reed ( Phragmites communis ) leaves from different habitats. Acta Bot Boreal Occident Sin, 2004, 24: 193-198.

  陈坤明,张承烈。干旱期间春小麦叶片多胺含量与作物抗旱性的关系。植物生理学报,2000,26(5):381-386.

  陈坤明,宫海军,陈国仓,王锁民,张承烈。春小麦不同发育阶段抗氧化系统对田间缓慢干旱的响应。西北植物学报,2003,23(9):1497-1505.

  陈坤明,宫海军,王锁民。植物抗坏血酸的生物合成、转运及生物学功能。西北植物学报,2004,24(2):329-336.

  陈坤明,宫海军,王锁民。植物谷胱甘肽代谢与环境胁迫。西北植物学报,2004,24(6):1119-1130.

  宫海军,陈坤明,王锁民,张承烈。植物硅营养的研究进展。西北植物学报, 2004,24(12):2385-2392.

  宫海军,陈坤明,陈国仓,王锁民,张承烈。春小麦叶片质膜氧化还原系统及其对缓慢干旱胁迫的响应。西北植物学报,2003,23(2):229-234.

  宫海军,陈坤明,陈国仓,朱学艺,王锁民,张承烈。缓慢干旱下春小麦叶片质膜脂肪酸组成、H+-ATPase及5’-AMPase活力的变化。植物生态学报,2003,27(4):459-465.

  宫海军,陈坤明,陈国仓,王锁民,张承烈。硅对小麦生长及其抗氧化酶系统的影响。土壤通报,2003,34(1):55-57.

  

  IV   研究生(Students)

  一、博士研究生 (Ph.D students)

             

  王响             卫小勇            常艳丽            景秀清            胡春红             巨岚         Muhammad Izhar

  二、硕士研究生(Master students)

                   

  孙良昱             姚林波               李彬彬               马田田             甘鹏飞                乔磊               袁博      

       

  史奕              台莉              李帅军                周梦茹