Rescue of an infection clone of barley yellow dwarf virus -GAV
Xingan Hao, Shuang Song, Qinrong Zhong, Jamal-U-Ddin Hajano, Jie Guo, Yunfeng Wu
Phytopathology
DOI: 10.1094/PHYTO-11-20-0522-R
Abstract
Barley yellow dwarf virus-GAV (BYDV-GAV) is one of the most prevalent viruses causing yellow dwarf disease in wheat in China. The biology and pathology of BYDV-GAV are well studied; however, gene functions and molecular mechanisms of BYDV-GAV disease development are unclear due to the lack of a reverse genetics system. In this study, a full-length cDNA clone of BYDV-GAV was constructed and expressed using Agrobacterium-mediated inoculation of Nicotiana benthamiana. Virions produced by BYDV-GAV in N. benthamiana were transmitted to wheat by an aphid vector after acquisition via a sandwich feeding method. Infectivity of the cDNA clone in wheat was verified through RT-PCR and Western blot assays, and the recombinant virus elicited typical reddening symptoms in oats and was transmitted between wheat plants. These results confirm the production of biologically active transmissible virions. Using the BYDV-GAV infectious clone, we demonstrate that the viral protein P4 was involved in cell-to-cell movement and stunting symptoms in wheat. This is the first report describing the development of an infectious full-length cDNA clone of BYDV-GAV and provides a useful tool for virus-host-vector interaction studies.