发表论文

Yong-Qiang Li, Li-Sha Bai, Cai-Xia Zhao, Jing-Jing Xu, Zhong-Juan Sun, Yan-Ling Dong, De-Xian Li, Xi-Li Liu, and Zhi-Qing Ma. Functional Characterization of Two Carboxylesterase Genes Involved in Pyrethroid Detoxification in Helicoverpa armigera

作者:  来源:doi: 10.1021/acs.jafc.9b06690  发布日期:2020-03-10  浏览次数:

Functional Characterization of Two Carboxylesterase Genes Involved in Pyrethroid Detoxification in Helicoverpa armigera

Yong-Qiang Li, Li-Sha Bai, Cai-Xia Zhao, Jing-Jing Xu, Zhong-Juan Sun, Yan-Ling Dong, De-Xian Li, Xi-Li Liu, and Zhi-Qing Ma



Agricultural and Food Chemistry

doi: 10.1021/acs.jafc.9b06690


Abstract: Insect carboxylesterases are major enzymes involved in metabolism of xenobiotics including insecticides. Two carboxylesterase genes, CarE001A and CarE001H, were cloned from the destructive agricultural pest Helicoverpa armigera. Quantitative real-time polymerase chain reaction showed that CarE001A and CarE001H were predominantly expressed in fat body and midgut, respectively; developmental expression analyses found that the expression levels of both CarEs were significantly higher in fifth-instar larvae than in other life stages. Recombinant CarE001A and CarE001H expressed in the Escherichia coli exhibited high enzymatic activity toward α-naphthyl acetate. Inhibition assays showed that organophosphates had strong inhibition on CarEs activity compared to pyrethroids. Metabolism assays indicated that CarE001A and CarE001H were able to metabolize βcypermethrin and λ-cyhalothrin. Homology modeling and molecular docking analyses demonstrated that β-cypermethrin could fit nicely into the active pocket of both carboxylesterases. These results suggested that CarE001A and CarE001H could play important roles in the detoxification of pyrehtroids in H. armigera.