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Yue Yin, Wei An, Jian-hua Zhao, Yan-long Li, Yun-fang Fan, Jin-huan Chen, You-long Cao and Xiang-qiang Zhan, p Constructing the wolfberry (L

作者:  来源:DOI: 10.1016/s2095-3119(21)63610-9  发布日期:2022-01-01  浏览次数:

p Constructing the wolfberry (Lycium spp.) genetic linkage map using AFLP and SSR markers

Yue Yin, Wei An, Jian-hua Zhao, Yan-long Li, Yun-fang Fan, Jin-huan Chen, You-long Cao and Xiang-qiang Zhan

Journal of Integrative Agriculture

DOI: 10.1016/s2095-3119(21)63610-9

Abstract

Genetic linkage maps are important for quantitative trait locus (QTL) and marker-assisted selection breeding. The wolfberry (Lycium spp.) is an important food and traditional medicine in China. However, few construction genetic linkage maps have been reported because of the lack of genomic and genetic resources. In this study, a population of 89 F1 seedings was derived from a cross between two heterozygous parents, L. chinense var. potaninii 'BF-01' (female) and L. barbarum var. auranticarpum 'NH-01' (male), in order to construct a genetic linkage map using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers based on the double pseudo-test cross mapping strategy. The resulting genetic map consisted of 165 markers (74 AFLPs and 91 SSRs) distributed across 12 linkage groups and spanned a total length of 557.6 cM with an average distance of 3.38 cM between adjacent markers. The 12 linkage groups contained 3 to 21 markers and ranged in length from 8.6 to 58.3 cM. Twenty-nine segregated markers distributed in the map were mainly located on LG4 and LG9 linkage groups at P<0.05. This is the first linkage map of Lycium species using SSR and AFLP markers, which can serve as basis for improving genes and selective breeding of the genome assembly.