Yinpeng Xie, Chana Bao, Pengxiang Chen, Fuguo Cao, Xiaofang Liu, Dali Geng, Zhongxing Li, Xuewei Li, Nan Hou, Fang Zhi, Chundong Niu, Shuangxi Zhou, Xiangqiang Zhan, Fengwang Ma, Qingmei Guan. Abscisic acid homeostasis is mediated by feedback regulation of MdMYB88 and MdMYB124

Abscisic acid homeostasis is mediated by feedback regulation of MdMYB88 and MdMYB124

Yinpeng Xie, Chana Bao, Pengxiang Chen, Fuguo Cao, Xiaofang Liu, Dali Geng, Zhongxing Li, Xuewei Li, Nan Hou, Fang Zhi, Chundong Niu, Shuangxi Zhou, Xiangqiang Zhan, Fengwang Ma, Qingmei Guan

Journal of Experimental Botany

DOI: 10.1093/jxb/eraa449

Abstract

The phytohormone abscisic acid (ABA) is involved in various plant processes. In response to drought stress, plants quickly accumulate ABA, but the regulatory mechanism of ABA accumulation is largely unknown, especially in woody plants. In this study, we report that MdMYB88 and MdMYB124 are myeloblastosis (MYB) transcription factors critical for ABA accumulation in apple trees (Malus x domestica) following drought, and this regulation is negatively controlled by ABA. MdMYB88 and MdMYB124 positively regulate leaf water transpiration, photosynthetic capacity, and stress endurance in apple trees under drought conditions. MdMYB88 and MdMYB124 regulate the expression of biosynthetic and catabolic genes of ABA, as well as drought- and ABA- responsive genes. MdMYB88 associates with promoter regions of the ABA biosynthetic gene 9-cis-epoxycarotenoid dioxygenase 3 (NCED3). Finally, expression of MdMYB88 and MdMYB124 is repressed by ABA. Our results identify a feedback regulation of MdMYB88 and MdMYB124 in modulating ABA homeostasis in apple trees.