Genome editing for plant research and crop improvement
Xiangqiang Zhan, Yuming Lu, Jian-Kang Zhu, Jose Ramon Botella
Journal of Integrative Plant Biology
The advent of CRISPR has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V and VI systems for gene disruption as well as for precise sequence alterations, gene transcription and epigenome control.