发表论文

Zhuo Wang, Tietao Wang, Rui Cui, Zhenxing Zhang, Keqi Chen, Mengyun Li, Yueyue Hua, Huawei Gu, Lei Xu, Yao Wang, Yantao Yang and Xihui Shen. HpaR, the Repressor of Aromatic Compound Metabolism, Positively Regulates the Expression of T6SS4 to Resist Oxidative Stress in Yersinia pseudotuberculosis

作者:  来源:doi: 10.3389/fmicb.2020.00705  发布日期:2020-04-17  浏览次数:

HpaR, the Repressor of Aromatic Compound Metabolism, Positively Regulates the Expression of T6SS4 to Resist Oxidative Stress in Yersinia pseudotuberculosis

Zhuo Wang, Tietao Wang, Rui Cui, Zhenxing Zhang, Keqi Chen, Mengyun Li, Yueyue Hua, Huawei Gu, Lei Xu, Yao Wang, Yantao Yang and Xihui Shen



Frontiers in Plant Science

doi: 10.3389/fmicb.2020.00705



Abstract:HpaR, a MarR family transcriptional regulator, was first identified in Escherichia coli W for its regulation of the hpa-meta operon. Little else is known regarding its functionality. Here, we report that in Yersinia pseudotuberculosis, HpaR negatively regulates the hpa-meta operon similar to in E. coli W. To investigate additional functions of HpaR, RNA sequencing was performed for both the wild-type and the 1hpaR mutant, which revealed that the type VI secretion system (T6SS) was positively regulated by HpaR. T6SS4 is important for bacteria resisting environmental stress, especially oxidative stress. We demonstrate that HpaR facilitates bacteria resist oxidative stress by upregulating the expression of T6SS4 in Y. pseudotuberculosis. HpaR is also involved in biofilm formation, antibiotic resistance, adhesion to eukaryotic cells, and virulence in mice. These results greatly expand our knowledge of the functionality of HpaR and reveal a new pathway that regulates T6SS4