Jia Zhou, Yuhua Wang, Xiaofei Liang, Changping Xie , Wenbo Liu, Weiguo Miao, Zhensheng Kang and Li Zheng . Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola

Molecular Characterization of a Novel Ourmia-Like Virus Infecting Phoma matteucciicola

Jia Zhou, Yuhua Wang, Xiaofei Liang, Changping Xie , Wenbo Liu, Weiguo Miao, Zhensheng Kang and Li Zheng

Viruses

doi: 10.3390/v12020231

Abstract: Here, we report a novel (+) ssRNA mycovirus, Phoma matteucciicola ourmia-like virus 1 (PmOLV1), isolated from Phoma matteucciicola strain LG915-1. The genome of PmOLV1 was 2603 nucleotides long and contained a single open reading frame (ORF), which could be translated into a product of RNA-dependent RNA polymerase (RdRp) by both standard and mitochondrial genetic codons. Cellular fractionation assay indicated that PmOLV1 RNAs are likely more enriched in mitochondria than in cytoplasm. Phylogenetic analysis indicated that PmOLV1 is a new member of the genus Penoulivirus (recently proposed) within the family Botourmiaviridae.