赵惠贤

  1. 基本信息

  赵惠贤,女, 三级教授,博士生导师;学校首届青年学术骨干支持计划入选者,中国生物化学与分子生物学学会农业分会第五届理事;在生物化学与分子生物学系从事分子生物学教学和研究工作。

  教育经历:

  1983.9-1987.6:西北农业大学土壤农化系攻读学士学位;

  1987.9-1990.6:西北农业大学植物生理生化专业攻读硕士学位;

  1999.9-2003.6:西北农林科技大学植物学专业在职攻读博士学位。

  进修经历:

  1994.10-1995.4:澳大利亚悉尼大学植物育种研究所进修小麦品质遗传育种;

  2005.10-2006.10:美国堪萨斯州立大学、美国农部农业研究中心进修小麦基因组和功能基因研究技术。

  

  2. 研究方向和研究内容

  ① 植物基因组学及功能基因研究:以小麦、油菜为主要研究对象,解析其品质和产量性状形成的分子基础及重要调控基因;

  ② 小麦抗旱分子生物学:小麦抗旱突变体的创制、筛选以及小麦响应干旱的分子基础研究。

  

  3. 教学工作

  承担本科生的《分子生物学》课程和研究生的《高级分子生物学》课程。

  

  4.研究课题

  主要开展调控小麦品质和产量功能基因分离和鉴定研究,目前承担研究项目主要包括:

  国家自然基金项目“2个小麦旗叶高表达的全新miRNA tae-miR5062和tae-miR9663的功能研究” (31501295)

  国家自然基金项目 “3个小麦种子特异表达的全新microRNA的功能研究(31471482)”

  高校基本科研业务费专项课题“小麦TaCYP78A3和TaCYP78A5基因的转基因改良初探”( Z109021568)

  高校基本科研业务费专项课题“小麦调控子粒大小基因TaCYP78A5的功能验证”( Z109021565)

  国家自然基金项目“低温诱导的小麦无选择标记基因遗传转化体系的建立”(31101205)

  

  5. 学术成就

  国家发明专利

  ① “用重组大麦条斑花叶病毒介导的小麦穗部和籽粒靶标基因沉默方法”专利授权公告日:2013.5.22,专利号:ZL201110450603.8;

  ② “建立油菜细胞质类型特异PCR标记以及快速鉴定油菜细胞质类型的方法” 专利授权公告日:2012.5.23,专利号: ZL 2009 1 0022297.0;

  ③ “小麦高分子量麦谷蛋白14亚基基因的核酸序列及其应用”专利授权公告日:2005.3.2,专利号: ZL02114579.2。

  

  获奖成果

  ①“小麦低分子量麦谷蛋白基因的克隆与功能鉴定” 2008年陕西省科学技术三等奖;

  ②“小麦低分子量麦谷蛋白基因的克隆与功能鉴定”2008年杨凌示范区科学技术一等奖;

  ③“陕西省主要农家种和改良种小麦加工品质鉴定”1999年度陕西省科技进步三等奖;

  ④“普通小麦稳定自交结实缺体系统的创制” 1998年国家发明三等奖。

  

  发表学术论文

  在The Plant Journal、Plant physiology、Journal of Experimental Botany、BMC Genomics、BMC Plant Biology、Theoretical and Applied Genetics等国际期刊发表论文30多篇;在作物学报、中国农业科学、农业生物技术学报等国内期刊发表论文50多篇。近期主要代表作如下:

  Ma Meng, Zhao Huixian(*), Li Zhaojie, Hu Shengwu, Song Weining, Liu Xiangli(*). TaCYP78A5 regulates seed size in wheat ( Triticum aestivum L. ). Journal of Experimental Botany, 2016, 65(5):1397-1410.

  Zhanjie Li, Peipei Zhang, JinyangLv,Yufeng Cheng,Jianmin Cui, Huixian Zhao(*), Shengwu Hu (*).Global Dynamic Transcriptome programming of metabolic processes of rapeseed ( Brassica napus L. ) anther at  different development stages. PLOS ONE, 2016,11(5): e0154039. doi:10.1371

  Meng Ma, Qian Wang, Zhanjie Li, Huihui Cheng, Zhaojie Li, Xiangli Liu, Weining Song, Rudi Appels and Huixian Zhao(*).Expression of TaCYP78A3 , a gene encoding cytochrome P450 CYP78A3 protein in wheat ( Triticum aestivum L. ), affects seed size. The Plant Journal, 2015,83:312-325.

  Xiaona Zhou, Hongmei Hao, Yuguo Zhang, Yili Bai, Wenbo Zhu, Yunxia Qin, Feifei Yuan, Feiyi Zhao, Mengyao Wang, Jingjiang Hu, Hong Xu, Aiguang Guo, Huixian Zhao, Yang Zhao, Cuiling Cao, Yongqing Yang, Karen S. Schumaker, Yan Guo and Chang Gen Xie(*). SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-type protein kinase, is important for abscisic acid responses in Arabidopsis through phosphorylation of ABSCISIC ACID-INSENSITIVE5. Plant Physiology , 2015, 168(2):659-676.

  Zhanjie Li, Yufeng Cheng, Jianmin Cui, Peipei Zhang, Huixian Zhao(*), & Shengwu Hu. Comparative transcriptome analysis reveals carbohydrate and lipid metabolism blocks in Brassica napus L. male sterility induced by the chemical hybridization agent monosulfuron ester sodium. BMC Genomics, 2015, 16:206 DOI 10.1186/s12864-015-1388-5

  Hui Liu, Muhammad Abdul Rab Faisal Sultan, Xiang li Liu, Jin Zhang, Fei Yu(*), Hui xian Zhao(*). Physiological and comparative proteomic analysis reveals different drought responses in roots and leaves of drought-tolerant wild wheat ( Triticum boeoticum ). PLOS ONE, 2015, 10(4):e0121852. doi:10.1371

  Ran Han, Chao Jian, Jinyang Lv, Yan Yan, Qing Chi, Zhanjie Li, Qian Wang, Jin Zhang, Xiangli Liu, Huixian Zhao(*). Identification and characterization of microRNAs in the flag leaf and developing seed of wheat (Triticum aestivum L.). BMC Genomics, 2014, 15:289.

  Yufeng Cheng, Qian Wang, Zhanjie Li, Jianmin Cui, Shengwu Hu(*), Huixian Zhao(*), Mingshun Chen. Cytological and comparative proteomic analyses on male sterility in Brassica napus L. induced by the chemical hybridization agent monosulphuron ester sodium. PLOS ONE, 2013,8 (11) e 80191.

  Han Ran, Yan Yan, Jian Chao, Zhao Hui xian (*). Comparison of two miRNA quantification methods for assaying expression profiles of miRNAs in wheat (Triticum aestivum L.). Journal of Integrative Agriculture.2014 13(4):733-740.

  Qian Wang, Wenda Huang, Qi Jiang, Jinpan Lian, Jianli Sun, Hong Xu, Huixian Zhao (*), Zhenlan Liu (*). Lower Levels of Expression of FATA2 Gene Promote Longer Siliques with Modified Seed Oil Content in Arabidopsis thaliana. Plant Molecular Biology Reporter, 2013,31(6):1368-1375.

  Muhammad Abdul Rab Faisal Sultan, Liu Hui, Cheng Yu Feng, Zhang Pei Pei, Zhao Hui Xian (*). Combining phytate/Ca2+ fractionation with trichloroacetic acid/acetone precipitation improved separation of low-abundant proteins of wheat (Triticum aestivum L.) leaf for proteomic analysis. Journal of Integrative Agriculture, 2013, 12(7):101-108.

  Meng Ma, Yan Yan, Li Huang, Mingshun Chen, Huixian Zhao (*). Virus-Induced Gene-Silencing in Wheat Spikes and Grains and Its Application in Functional Analysis of HMW-GS-encoding Genes. BMC Plant biology, 2012,12:141. (IF2012: 4.354)

  Muhammad Abdul Rab Faisal Sultan, Liu Hui, Lv Jin Yang, Zhao Huixian (*). Assessment of Drought Tolerance of Some Triticum L. Species through Physiological Indices. Czech Journal of Genetics and Plant Breeding, 2012, 48(4): 178–184.

  Huixian Zhao, Zhanjie Li, Shengwu Hu ( * ), Genlou Sun, Jianjun Chang. Identification of cytoplasm types in rapeseed (Brassica napus L.) accessions by a multiplex PCR assay. Theor.Appl.Genet, 2010,121:643–650.

  Ming-Shun Chen (*), Xuming Liu,Ziheng Yang,Hui-Xian Zhao,Richard H Shukle,Jeffrey J Stuart. Unusual Conservation among Genes Encoding Small Secreted Salivary Gland Proteins from a Gall Midge. BMC Evolutionary Biology, 2010, 10:296.

  

  联系方式:

  通讯地址:陕西杨凌邰城路3号 西北农林科技大学生命科学学院

  邮编:712100

  电话:029-87092262

   E-mail: hxzhao212@nwsuaf.edu.cn