Venturicidin A Is a Potential Fungicide for Controlling Fusarium Head Blight by Affecting Deoxynivalenol Biosynthesis, Toxisome Formation, and Mitochondrial Structure
Hu, Lifang, Cong Guo, Jing Chen, Ruimin Jia, Yan Sun, Shang Cao, Ping Xiang and Yang Wang
Journal of agricultural and food chemistry
https://doi.org/10.1021/acs.jafc.3c02683
ABSTRACT
Fusarium graminearum,which causes Fusarium head blight (FHB) in cereals,is one of the mostdevastating fungal diseases by causing great yield losses and mycotoxincontamination. A major bioactive ingredient, venturicidin A (VentA),was isolated from Streptomyces pratensis S10 mycelial extract with an activity-guided approach. No reportis available on antifungal activity of VentA against F. graminearum and effects on deoxynivalenol (DON)biosynthesis. Here, VentA showed a high antagonistic activity toward F. graminearum with an EC50 value of 3.69 & mu;g/mL. As observed by scanning electron microscopy, after exposureto VentA, F. graminearum conidia andmycelia appeared abnormal. Different dyes staining revealed that VentAincreased cell membrane permeability. In growth chamber and fieldtrials, VentA effectively reduced disease severity of FHB. Moreover,VentA inhibited DON biosynthesis by reducing pyruvic acid, acetyl-CoAproduction, and accumulation of reactive oxygen species (ROS) andthen inhibiting trichothecene (TRI) genes expressionand toxisome formation. These results suggest that VentA is a potentialfungicide for controlling FHB.